Isolation and Characterization of theHeat - responsiyeGenes in Escherichia coti
نویسندگان
چکیده
K-12. Furthermore, a new gene (hrsA) that suppressed the mutation was cloned. Its nuc]eotide sequence -'as analyzed and it was located close to the suc operon at 16.7min corresponding to #18Fl1 (Kohara bank) on E. coti genome. In TD2 containing the hrsA on a mlllticopy plasmid, the ompC expression was induced and dependent on OmpR with increased temperature. The HrsA was found to have Enzyme IIA, IIB, and IIC domains that are homologous to Enzyme Il, inyo]yed in the fructose-specific PTS (phosphotransferase system). The putative phosphorylation sites (His87 and Cys192) were also conserved in HrsA.
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